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Braz. j. microbiol ; 40(2): 394-398, Apr.-June 2009. ilus, tab
Article in English | LILACS | ID: lil-520229

ABSTRACT

In this study we investigated the phenotypic slime production of Vibrio alginolyticus and Vibrioparahaemolyticus strains, food-borne pathogens, using a Congo red agar plate assay. Furthermore, westudied their ability to adhere to abiotic surfaces and Vero cells line. Our results showed that only V.alginolyticus ATCC 17749 was a slime-producer developing almost black colonies on Congo red agar plate.Adherence to glace tube showed that all V. alginolyticus strains were more adherent than V. parahaemolyticus.Only V. alginolyticus ATCC 17749 was found to be able to form biofilm on polystyrene microplate wells (OD570= 0.532). Adherence to Vero cells showed that all tested strains were non adherent after 30 min, however after60 min all the studied strains become adherent. The percentage of adherence ranged from1.23% to 4.66%.


Neste estudo, investigou-se a produção de muco por cepas de Vibrio alginolyticus e Vibrio parahaemolyticus através do teste em placa de ágar com vermelho congo. Estudou-se também a capacidade de adesão à superfícies abióticas e células Vero. Os resultados indicaram que somente V. alginolyticus ATCC 17749 produziu muco, formando colônias quase negras nas placas de ágar com vermelho congo. O teste de adesão a tubos de vidro indicou que as cepas de V. alginolyticus foram maisaderentes do que as de V. parahaemolyticus. Somente V. alginolyticus ATCC 17749 foi capaz de formar biofilme nos poços das microplacas de poliestireno (OD570=0,532). Testes de adesão a células Vero mostraram que nenhuma das cepas apresentou adesão em 30 min, mas todas aderiram após 60 min. Aporcentagem de adesão variou de 1,23% a 4,66%.


Subject(s)
Focal Adhesions , Bacterial Adhesion , Biofilms/growth & development , Mucus , Congo Red/analysis , Vibrio alginolyticus/isolation & purification , Agar , Methods , Methods
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